|
週次 |
日期 |
單元主題 |
|
第1週 |
2/20 |
Overview |
|
第2週 |
|
Generation of Targets for Spotted Microarrays
(Inoculate, miniprep and DNA gel electrophoresis) |
|
第3週 |
|
Generation of Targets for Spotted Microarrays (PCR and spectrophotometer) |
|
第4週 |
|
Generation of Targets for Spotted Microarrays (Plate transfer, Beckman FX) |
|
第5週 |
|
Printing Spotted Glass Microarrays (Printing design (OmniGrid arrayer)) |
|
第6週 |
|
Printing Spotted Glass Microarrays (Post-processing) |
|
第7週 |
4/3 |
Spring break (no class) |
|
第8週 |
4/10 |
Expression Analysis of RNA (Cell culture and RNA extraction) Class starts at 1:30. |
|
第9週 |
4/17 |
Midterm |
|
第10週 |
4/24 |
No class |
|
第11週 |
5/1 |
No class |
|
第12週 |
5/8 |
RNA purification and quality check |
|
第13週 |
5/15 |
Microarray Bioinformatics
(Illumina and Affymetrix system intro) |
|
第14週 |
5/22 |
Microarray Bioinformatics (Real-time RT PCR) |
|
第15週 |
5/29 |
Expression analysis of RNA (cDNA labelling and hybridization, 5/31-6/1) |
|
第16週 |
6/5 |
Microarray Bioinformatics (Gal file preparation and Scan image) |
|
第17週 |
6/12 |
Microarray Bioinformatics (Data analysis and IPA intro) |
|
第18週 |
|
Final exam (MD203 at 2pm) |